Which of the following reagents is used to differentiate between IgM and IgG antibodies in hemagglutination reactions?

A Dithiothreitol (DTT)
B 2-Mercaptoethanol (2-ME) ✓
C Sulfhydryl compounds
D Enzyme-treated red cells

Explanation

2-Mercaptoethanol (2-ME) is used to differentiate between IgM and IgG antibodies as it reduces disulfide bonds in IgM, preventing it from participating in hemagglutination reactions.

Source

ISBT Science Series
Hematology Hematology: Blood Grouping and Crossmatching Hematology: Blood Grouping and Crossmatching Hard MEDICAL Confidence: high