Preparation of Packed Red Cells

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Packed Red blood cells, also known as PRBCs, Concentrated RBCs or simply "Packed cells", are a type of blood component used for blood transfusions. Packed red blood cells are typically given in situations where the patient has either lost a large amount of blood or has severed anemia (Haemoglobin level below 6 gm/dl) that is causing notable symptoms.

Preparation of Packed Red Cells

Using double bags:

  1. Process all units within 6 hours of blood collection.
  2. Keep units vertical on laminar flow table for 30 – 45 minutes.
  3. Keep bags in buckets and balance them. Keep equally balanced buckets with bags diagonally opposite in refrigerator centrifuge ensuring that the poison of bags in buckets is parallel to direction of spin.
  4. Centrifuge at 4ºC at 3880 r.p.m. for 5 minutes.
  5. After centrifugation gently remove the bags form buckets and placed them on Expresser stand under laminar flow. Break the integral seal connecting satellite bags. Manually express supernatant plasma in the satellite bag.
  6. In case of double bags leave 50 – 60 ml of plasma bark along with red cells in primary bag. This component is Packed Red Cells and. stored it at 4°C (2°C - 8°C) temperature in the refrigerator and kept in component room.
  7. Label the bags and take it on inventory after testing is over.

Using Triple bags with or without additive solution:

  1. Process the blood collected within 6 hours of time of collection.
  2. Keep units vertical on laminar flow table for 30 – 45 minutes.
  3. Note the weight of primary bag and record in register.
  4. Keep bags in buckets and balance them.
  5. Keep equally balanced bag diagonally opposite in refrigerated centrifuge ensuring that the position of bags in bucket is paralleled to direction of spin.
  6. Centrifuge at 3500 r.p.m. for 10 minutes at 4ºC.
  7. Keep bag on plasma separation on laminar flow. Break the seal of tubing connecting satellite bag. Express plasma in satellite bag leaving 50 – 60 ml of plasma in primary bag.  If additive is used, remove all plasma in satellite bag before clamping.
  8. Remove the clamp of bag containing additive solution and let additive solution pass slowly in primary bag containing Red cells.
  9. Mixed contain thoroughly, Seal the tubing and Detach the bag.
  10. Keep primary bag containing Packed cells with additive solution in quarantine storage in blood bank at 4ºC (2ºC - 8ºC) in the refrigerator and kept in component room.
  11. Label the bags and take it on inventory after testing is over.

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