Which of the following glycolytic enzymes is inhibited by high concentrations of ATP, citrate, and NADH?

A Hexokinase
B Phosphofructokinase-1 ✓
C Pyruvate kinase
D Glyceraldehyde-3-phosphate dehydrogenase

Explanation

Phosphofructokinase-1 is a key regulatory enzyme in glycolysis that is inhibited by high concentrations of ATP, citrate, and NADH, which signal a sufficient energy status in the cell.

Mnemonic

_PFK-1_ → '1' key regulator of glycolysis, inhibited by 'high' energy signals

Source

Stryer Biochemistry
Carbohydrate Metabolism Biochemistry Glycolysis Regulation Hard MEDICAL Confidence: high