What is the purpose of using a two-stage crossmatching technique in blood banking?

A To detect warm autoantibodies
B To differentiate between ABO and Rh incompatibility
C To reduce the risk of transfusion-related acute lung injury (TRALI)
D To identify the presence of alloantibodies against red blood cell antigens
Two-stage crossmatching is used to identify the presence of alloantibodies against red blood cell antigens, which can help prevent hemolytic transfusion reactions.
AABB Technical Manual
Hematology Hematology: Blood Grouping and Crossmatching Hematology: Blood Grouping and Crossmatching Medium MEDICAL Confidence: high