What is the purpose of using a 'two-stage' crossmatch procedure in pre-transfusion testing?

A To detect antibodies against platelet antigens
B To identify antibodies against white blood cell antigens
C To distinguish between IgM and IgG antibodies against red blood cell antigens
D To enhance the sensitivity of antibody detection against red blood cell antigens ✓

Explanation

The two-stage crossmatch procedure involves an initial screening for antibodies at room temperature, followed by an incubation step at 37°C, which enhances the sensitivity of antibody detection against red blood cell antigens.

Source

Transfusion Medicine: Clinical Principles and Practice
Hematology Hematology: Blood Grouping and Crossmatching Hematology: Blood Grouping and Crossmatching Hard MEDICAL Confidence: medium