What is the primary mechanism by which 2,3-Bisphosphoglycerate regulates hemoglobin's oxygen binding affinity?

A Increasing the affinity of hemoglobin for oxygen
B Decreasing the affinity of hemoglobin for oxygen ✓
C Enhancing the binding of nitric oxide to hemoglobin
D Inhibiting the release of oxygen from hemoglobin to tissues

Explanation

2,3-Bisphosphoglycerate binds to deoxyhemoglobin, stabilizing the T-state and decreasing the affinity of hemoglobin for oxygen, facilitating oxygen release to tissues.

Source

Guyton and Hall Textbook of Medical Physiology
Hematology Hematology: Hemoglobin and Oxygen Transport Hematology: Hemoglobin and Oxygen Transport Medium MEDICAL Confidence: high